The new detection was made near Nana Glen, north-west of Coffs Harbour, exactly a month after the deadly honeybee parasite was first discovered on Australian soil.
The find has lead to another set of biosecurity zones being established, the Department of Primary Industries (DPI) says.
The DPI told 9news.com.au that as of 6pm last night, 2487 hives had been euthanized statewide to prevent the mites spreading further.
All hives on infected properties will be humanely euthanized, and the movement of both the hives and equipment over the past 12 months will be tracked in a bid to slow the spread of the parasite.
Member for Coffs Harbor Gurmesh Singh says a widespread outbreak would have significant impact on local produce.
“Bees play such an important role as pollinators and they drastically improve the quality of the fruit and vegetables that we consume every day,” Singh said.
“The quicker we can get things under control the better.
“I’m encouraging farmers and apiarists to please come forward and report the locations of your hives, as it is an invaluable component of our control measures.”
NSW Farmers Association said the mite is causing particular concern among blueberry and avocado growers.
“It’s a bit of a worry for our farmers as we approach pollination time, without bees we can’t produce avocados or blueberries, or a lot of other crops for that matter,” Coffs Harbor Branch Chair Paul Shoker said.
“We understand the Department of Primary Industries and Local Land Services are doing their part to keep the outbreak under control, we just need everyone else to do the right thing as well.
“People should continue to report the locations of any hives, both managed hives and wild hives, they might be aware of.”
Despite the discovery, Minister for Agriculture Dugald Saunders maintains the DPI has “a good handle on the situation”.
“The good news is we can still draw a direct line between every single case so far,” he said.
“Our tracing efforts have led us to this infested premise after hives were moved earlier this year, prior to the introduction of the state-wide pause on movement.
“Our field officers are working hard to conduct hive inspections with beekeepers right across the State and have connected this incursion to an existing case in the Hunter cluster.”
A statewide standstill of hives is currently in place, however movement from the general biosecurity emergency zone is being allowed under a permit-based system.
All beekeepers seeking to move bees and beehives in low-risk areas are encouraged to complete a Hive Movement Declaration.
“We have opened a safe and traceable way to allow the crucial movement of honeybees and apiary equipment and allow for the honey and pollination season to get underway while minimizing risk of any further spread,” NSW DPI Chief Plant Protection Officer Dr Satendra Kumar said.
Invisible to the naked eye, it’s one of Australia’s biggest kilers
Bee industry under threat from tiny parasite
Experts have warned a widespread outbreak could cost the honey industry $70 million a year if not contained.
Within the first nineteen days the DPI had euthanized at least 1693 hives in various kill zones across parts of the state.
Estimates suggest each hive is home to between 10,000 – 30,000 bees.
Until this point the pest, considered the most serious for honey bees worldwide, had not been found in Australia.
The parasites cling to honeybees as they feed and spreads debilitating viruses like deformed wing viruses.